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Chromatography Lab

Uploaded by Gotskillz on Dec 21, 2004

Title: Chromatography Lab

Purpose (Hypothesis): To understand the process of chromatography, and see which color will have the greatest rate of seperation.

Materials:

Nine drops of blue dye; Fifteen drops of green dye, Twenty seven drops of yellow, Seventy drops of re-orange, 250 ml beaker, Absorbent, Water, Pipette

Procedures:

Gather Materials  Fill Beaker W with approximately 50 milliliters of water  Fill Beaker A with approximately 50 milliliters of alcohol  Cut two pieces of absorbent paper with an approximate length of 15 centimeters each  Using the pipette, squeeze one drop of the mixed dyes onto both absorbent papers high enough so that when they are placed in the beakers, the dyes will not touch the fluids  Allow chromatography to take place over an extended period of time and record the results

Data:

Dyes in Water Rate
Blue .0404 mm/seconds
Green .0346 mm/seconds
Yellow .0334 mm/seconds

Dyes in Alcohol Rate
Blue .0129 mm/seconds
Green .0102 mm/seconds
Yellow .0099 mm/seconds

Conclusion:

Chromatography, which is employed to split-up organic compounds from mixtures on the basis of their solubility in a solvent, is demonstrated in this lab. Chromatography takes place when the absorbent paper is placed in the two substances. After an extended period of time, the organic compounds in the mixtures break up at different rates, depending on their solubility in the water or alcohol. Blue had the greatest rate of separation in both substances, followed by green, and yellow. The mixture in the water moved up the paper about 3 times as fast as the same mixture in alcohol. Chromatography, which is employed to split-up organic compounds from mixtures on the basis of their solubility in a solvent, is demonstrated in this lab. Chromatography takes place when the absorbent paper is placed in the two substances. After an extended period of time, the organic compounds in the mixtures break up at different rates, depending on their solubility in the water or alcohol. Blue had the greatest rate of separation in both substances, followed by green, and yellow. The mixture in the water moved up the paper about 3 times as fast as the same mixture in alcohol.

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Uploaded by:   Gotskillz

Date:   12/21/2004

Category:   Biology

Length:   2 pages (339 words)

Views:   11692

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